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gel supershift  (R&D Systems)


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    Structured Review

    R&D Systems gel supershift
    Gel Supershift, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gel+supershift/Human+Carbonic+Anhydrase+IX%2FCA9+Antibody/pmc08825433__10555_2021_10011_MOESM1_ESM-3-93-95
    Average 93 stars, based on 32 article reviews
    gel supershift - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Western Blot:

    Article Title: Understanding metabolic alterations and heterogeneity in cancer progression through validated immunodetection of key molecular components: a case of carbonic anhydrase IX
    Article Snippet: Name Host Antibody type Immunogen/epitope Key applications M75 M monoclonal PG region WB, FACS, IP, IF, IHC, ELISA Abcam ab15086 R polyclonal synthetic peptide (aa 359-459) WB, FACS, IHC Cell Signaling Technology D10C10 R monoclonal synthetic peptide (around Asp361) WB, IHC Invitrogen/ThermoFisher Scientific GT12 M monoclonal linear repetitive epitope in the PG region WB, FACS, IF, ICC, IHC, IP Merck 2D3 M monoclonal purified recombinant fragment expressed in E. coli WB, FACS, IHC Novus Biologicals NB100417 R polyclonal synthetic peptide from the Cterminal region (aa 400-459) WB, FACS, IF, ICC, IHC, ELISA, ChIP, Gel Supershift, R&D Systems AF2188 G polyclonal mouse myeloma cell line NS0 derived recombinant protein Pro59 - Asp414 WB, FACS, ICC, IHC, IP Santa Cruz Biotechnology H-11 M monoclonal aa 41-160 WB, IP, IF, IHC, ELISA Sigma-Aldrich SAB1300310 R polyclonal N-terminal region WB, ELISA M – mouse, R – rabbit, G – goat, WB – Wester blotting, FACS – fluorescence-activated cell sorting, IHC – immunohistochemistry, IF – immunofluorescence, ICC – immunocytochemistry, ELISA – enzyme-linked immunosorbent assay, IP – immunoprecipitation, ChIP – chromatin immunoprecipitation Supplementary Table S2: Dilutions of all anti-CA IX antibodies selected on the basis of manufacturers’ recommendation and authors’ experience.

    FACS:

    Article Title: Understanding metabolic alterations and heterogeneity in cancer progression through validated immunodetection of key molecular components: a case of carbonic anhydrase IX
    Article Snippet: Name Host Antibody type Immunogen/epitope Key applications M75 M monoclonal PG region WB, FACS, IP, IF, IHC, ELISA Abcam ab15086 R polyclonal synthetic peptide (aa 359-459) WB, FACS, IHC Cell Signaling Technology D10C10 R monoclonal synthetic peptide (around Asp361) WB, IHC Invitrogen/ThermoFisher Scientific GT12 M monoclonal linear repetitive epitope in the PG region WB, FACS, IF, ICC, IHC, IP Merck 2D3 M monoclonal purified recombinant fragment expressed in E. coli WB, FACS, IHC Novus Biologicals NB100417 R polyclonal synthetic peptide from the Cterminal region (aa 400-459) WB, FACS, IF, ICC, IHC, ELISA, ChIP, Gel Supershift, R&D Systems AF2188 G polyclonal mouse myeloma cell line NS0 derived recombinant protein Pro59 - Asp414 WB, FACS, ICC, IHC, IP Santa Cruz Biotechnology H-11 M monoclonal aa 41-160 WB, IP, IF, IHC, ELISA Sigma-Aldrich SAB1300310 R polyclonal N-terminal region WB, ELISA M – mouse, R – rabbit, G – goat, WB – Wester blotting, FACS – fluorescence-activated cell sorting, IHC – immunohistochemistry, IF – immunofluorescence, ICC – immunocytochemistry, ELISA – enzyme-linked immunosorbent assay, IP – immunoprecipitation, ChIP – chromatin immunoprecipitation Supplementary Table S2: Dilutions of all anti-CA IX antibodies selected on the basis of manufacturers’ recommendation and authors’ experience.

    Immunohistochemistry:

    Article Title: Understanding metabolic alterations and heterogeneity in cancer progression through validated immunodetection of key molecular components: a case of carbonic anhydrase IX
    Article Snippet: Name Host Antibody type Immunogen/epitope Key applications M75 M monoclonal PG region WB, FACS, IP, IF, IHC, ELISA Abcam ab15086 R polyclonal synthetic peptide (aa 359-459) WB, FACS, IHC Cell Signaling Technology D10C10 R monoclonal synthetic peptide (around Asp361) WB, IHC Invitrogen/ThermoFisher Scientific GT12 M monoclonal linear repetitive epitope in the PG region WB, FACS, IF, ICC, IHC, IP Merck 2D3 M monoclonal purified recombinant fragment expressed in E. coli WB, FACS, IHC Novus Biologicals NB100417 R polyclonal synthetic peptide from the Cterminal region (aa 400-459) WB, FACS, IF, ICC, IHC, ELISA, ChIP, Gel Supershift, R&D Systems AF2188 G polyclonal mouse myeloma cell line NS0 derived recombinant protein Pro59 - Asp414 WB, FACS, ICC, IHC, IP Santa Cruz Biotechnology H-11 M monoclonal aa 41-160 WB, IP, IF, IHC, ELISA Sigma-Aldrich SAB1300310 R polyclonal N-terminal region WB, ELISA M – mouse, R – rabbit, G – goat, WB – Wester blotting, FACS – fluorescence-activated cell sorting, IHC – immunohistochemistry, IF – immunofluorescence, ICC – immunocytochemistry, ELISA – enzyme-linked immunosorbent assay, IP – immunoprecipitation, ChIP – chromatin immunoprecipitation Supplementary Table S2: Dilutions of all anti-CA IX antibodies selected on the basis of manufacturers’ recommendation and authors’ experience.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Understanding metabolic alterations and heterogeneity in cancer progression through validated immunodetection of key molecular components: a case of carbonic anhydrase IX
    Article Snippet: Name Host Antibody type Immunogen/epitope Key applications M75 M monoclonal PG region WB, FACS, IP, IF, IHC, ELISA Abcam ab15086 R polyclonal synthetic peptide (aa 359-459) WB, FACS, IHC Cell Signaling Technology D10C10 R monoclonal synthetic peptide (around Asp361) WB, IHC Invitrogen/ThermoFisher Scientific GT12 M monoclonal linear repetitive epitope in the PG region WB, FACS, IF, ICC, IHC, IP Merck 2D3 M monoclonal purified recombinant fragment expressed in E. coli WB, FACS, IHC Novus Biologicals NB100417 R polyclonal synthetic peptide from the Cterminal region (aa 400-459) WB, FACS, IF, ICC, IHC, ELISA, ChIP, Gel Supershift, R&D Systems AF2188 G polyclonal mouse myeloma cell line NS0 derived recombinant protein Pro59 - Asp414 WB, FACS, ICC, IHC, IP Santa Cruz Biotechnology H-11 M monoclonal aa 41-160 WB, IP, IF, IHC, ELISA Sigma-Aldrich SAB1300310 R polyclonal N-terminal region WB, ELISA M – mouse, R – rabbit, G – goat, WB – Wester blotting, FACS – fluorescence-activated cell sorting, IHC – immunohistochemistry, IF – immunofluorescence, ICC – immunocytochemistry, ELISA – enzyme-linked immunosorbent assay, IP – immunoprecipitation, ChIP – chromatin immunoprecipitation Supplementary Table S2: Dilutions of all anti-CA IX antibodies selected on the basis of manufacturers’ recommendation and authors’ experience.

    Immunocytochemistry:

    Article Title: Understanding metabolic alterations and heterogeneity in cancer progression through validated immunodetection of key molecular components: a case of carbonic anhydrase IX
    Article Snippet: Name Host Antibody type Immunogen/epitope Key applications M75 M monoclonal PG region WB, FACS, IP, IF, IHC, ELISA Abcam ab15086 R polyclonal synthetic peptide (aa 359-459) WB, FACS, IHC Cell Signaling Technology D10C10 R monoclonal synthetic peptide (around Asp361) WB, IHC Invitrogen/ThermoFisher Scientific GT12 M monoclonal linear repetitive epitope in the PG region WB, FACS, IF, ICC, IHC, IP Merck 2D3 M monoclonal purified recombinant fragment expressed in E. coli WB, FACS, IHC Novus Biologicals NB100417 R polyclonal synthetic peptide from the Cterminal region (aa 400-459) WB, FACS, IF, ICC, IHC, ELISA, ChIP, Gel Supershift, R&D Systems AF2188 G polyclonal mouse myeloma cell line NS0 derived recombinant protein Pro59 - Asp414 WB, FACS, ICC, IHC, IP Santa Cruz Biotechnology H-11 M monoclonal aa 41-160 WB, IP, IF, IHC, ELISA Sigma-Aldrich SAB1300310 R polyclonal N-terminal region WB, ELISA M – mouse, R – rabbit, G – goat, WB – Wester blotting, FACS – fluorescence-activated cell sorting, IHC – immunohistochemistry, IF – immunofluorescence, ICC – immunocytochemistry, ELISA – enzyme-linked immunosorbent assay, IP – immunoprecipitation, ChIP – chromatin immunoprecipitation Supplementary Table S2: Dilutions of all anti-CA IX antibodies selected on the basis of manufacturers’ recommendation and authors’ experience.

    Purification:

    Article Title: Understanding metabolic alterations and heterogeneity in cancer progression through validated immunodetection of key molecular components: a case of carbonic anhydrase IX
    Article Snippet: Name Host Antibody type Immunogen/epitope Key applications M75 M monoclonal PG region WB, FACS, IP, IF, IHC, ELISA Abcam ab15086 R polyclonal synthetic peptide (aa 359-459) WB, FACS, IHC Cell Signaling Technology D10C10 R monoclonal synthetic peptide (around Asp361) WB, IHC Invitrogen/ThermoFisher Scientific GT12 M monoclonal linear repetitive epitope in the PG region WB, FACS, IF, ICC, IHC, IP Merck 2D3 M monoclonal purified recombinant fragment expressed in E. coli WB, FACS, IHC Novus Biologicals NB100417 R polyclonal synthetic peptide from the Cterminal region (aa 400-459) WB, FACS, IF, ICC, IHC, ELISA, ChIP, Gel Supershift, R&D Systems AF2188 G polyclonal mouse myeloma cell line NS0 derived recombinant protein Pro59 - Asp414 WB, FACS, ICC, IHC, IP Santa Cruz Biotechnology H-11 M monoclonal aa 41-160 WB, IP, IF, IHC, ELISA Sigma-Aldrich SAB1300310 R polyclonal N-terminal region WB, ELISA M – mouse, R – rabbit, G – goat, WB – Wester blotting, FACS – fluorescence-activated cell sorting, IHC – immunohistochemistry, IF – immunofluorescence, ICC – immunocytochemistry, ELISA – enzyme-linked immunosorbent assay, IP – immunoprecipitation, ChIP – chromatin immunoprecipitation Supplementary Table S2: Dilutions of all anti-CA IX antibodies selected on the basis of manufacturers’ recommendation and authors’ experience.

    Recombinant:

    Article Title: Understanding metabolic alterations and heterogeneity in cancer progression through validated immunodetection of key molecular components: a case of carbonic anhydrase IX
    Article Snippet: Name Host Antibody type Immunogen/epitope Key applications M75 M monoclonal PG region WB, FACS, IP, IF, IHC, ELISA Abcam ab15086 R polyclonal synthetic peptide (aa 359-459) WB, FACS, IHC Cell Signaling Technology D10C10 R monoclonal synthetic peptide (around Asp361) WB, IHC Invitrogen/ThermoFisher Scientific GT12 M monoclonal linear repetitive epitope in the PG region WB, FACS, IF, ICC, IHC, IP Merck 2D3 M monoclonal purified recombinant fragment expressed in E. coli WB, FACS, IHC Novus Biologicals NB100417 R polyclonal synthetic peptide from the Cterminal region (aa 400-459) WB, FACS, IF, ICC, IHC, ELISA, ChIP, Gel Supershift, R&D Systems AF2188 G polyclonal mouse myeloma cell line NS0 derived recombinant protein Pro59 - Asp414 WB, FACS, ICC, IHC, IP Santa Cruz Biotechnology H-11 M monoclonal aa 41-160 WB, IP, IF, IHC, ELISA Sigma-Aldrich SAB1300310 R polyclonal N-terminal region WB, ELISA M – mouse, R – rabbit, G – goat, WB – Wester blotting, FACS – fluorescence-activated cell sorting, IHC – immunohistochemistry, IF – immunofluorescence, ICC – immunocytochemistry, ELISA – enzyme-linked immunosorbent assay, IP – immunoprecipitation, ChIP – chromatin immunoprecipitation Supplementary Table S2: Dilutions of all anti-CA IX antibodies selected on the basis of manufacturers’ recommendation and authors’ experience.

    Chromatin Immunoprecipitation:

    Article Title: Understanding metabolic alterations and heterogeneity in cancer progression through validated immunodetection of key molecular components: a case of carbonic anhydrase IX
    Article Snippet: Name Host Antibody type Immunogen/epitope Key applications M75 M monoclonal PG region WB, FACS, IP, IF, IHC, ELISA Abcam ab15086 R polyclonal synthetic peptide (aa 359-459) WB, FACS, IHC Cell Signaling Technology D10C10 R monoclonal synthetic peptide (around Asp361) WB, IHC Invitrogen/ThermoFisher Scientific GT12 M monoclonal linear repetitive epitope in the PG region WB, FACS, IF, ICC, IHC, IP Merck 2D3 M monoclonal purified recombinant fragment expressed in E. coli WB, FACS, IHC Novus Biologicals NB100417 R polyclonal synthetic peptide from the Cterminal region (aa 400-459) WB, FACS, IF, ICC, IHC, ELISA, ChIP, Gel Supershift, R&D Systems AF2188 G polyclonal mouse myeloma cell line NS0 derived recombinant protein Pro59 - Asp414 WB, FACS, ICC, IHC, IP Santa Cruz Biotechnology H-11 M monoclonal aa 41-160 WB, IP, IF, IHC, ELISA Sigma-Aldrich SAB1300310 R polyclonal N-terminal region WB, ELISA M – mouse, R – rabbit, G – goat, WB – Wester blotting, FACS – fluorescence-activated cell sorting, IHC – immunohistochemistry, IF – immunofluorescence, ICC – immunocytochemistry, ELISA – enzyme-linked immunosorbent assay, IP – immunoprecipitation, ChIP – chromatin immunoprecipitation Supplementary Table S2: Dilutions of all anti-CA IX antibodies selected on the basis of manufacturers’ recommendation and authors’ experience.

    Derivative Assay:

    Article Title: Understanding metabolic alterations and heterogeneity in cancer progression through validated immunodetection of key molecular components: a case of carbonic anhydrase IX
    Article Snippet: Name Host Antibody type Immunogen/epitope Key applications M75 M monoclonal PG region WB, FACS, IP, IF, IHC, ELISA Abcam ab15086 R polyclonal synthetic peptide (aa 359-459) WB, FACS, IHC Cell Signaling Technology D10C10 R monoclonal synthetic peptide (around Asp361) WB, IHC Invitrogen/ThermoFisher Scientific GT12 M monoclonal linear repetitive epitope in the PG region WB, FACS, IF, ICC, IHC, IP Merck 2D3 M monoclonal purified recombinant fragment expressed in E. coli WB, FACS, IHC Novus Biologicals NB100417 R polyclonal synthetic peptide from the Cterminal region (aa 400-459) WB, FACS, IF, ICC, IHC, ELISA, ChIP, Gel Supershift, R&D Systems AF2188 G polyclonal mouse myeloma cell line NS0 derived recombinant protein Pro59 - Asp414 WB, FACS, ICC, IHC, IP Santa Cruz Biotechnology H-11 M monoclonal aa 41-160 WB, IP, IF, IHC, ELISA Sigma-Aldrich SAB1300310 R polyclonal N-terminal region WB, ELISA M – mouse, R – rabbit, G – goat, WB – Wester blotting, FACS – fluorescence-activated cell sorting, IHC – immunohistochemistry, IF – immunofluorescence, ICC – immunocytochemistry, ELISA – enzyme-linked immunosorbent assay, IP – immunoprecipitation, ChIP – chromatin immunoprecipitation Supplementary Table S2: Dilutions of all anti-CA IX antibodies selected on the basis of manufacturers’ recommendation and authors’ experience.

    Fluorescence:

    Article Title: Understanding metabolic alterations and heterogeneity in cancer progression through validated immunodetection of key molecular components: a case of carbonic anhydrase IX
    Article Snippet: Name Host Antibody type Immunogen/epitope Key applications M75 M monoclonal PG region WB, FACS, IP, IF, IHC, ELISA Abcam ab15086 R polyclonal synthetic peptide (aa 359-459) WB, FACS, IHC Cell Signaling Technology D10C10 R monoclonal synthetic peptide (around Asp361) WB, IHC Invitrogen/ThermoFisher Scientific GT12 M monoclonal linear repetitive epitope in the PG region WB, FACS, IF, ICC, IHC, IP Merck 2D3 M monoclonal purified recombinant fragment expressed in E. coli WB, FACS, IHC Novus Biologicals NB100417 R polyclonal synthetic peptide from the Cterminal region (aa 400-459) WB, FACS, IF, ICC, IHC, ELISA, ChIP, Gel Supershift, R&D Systems AF2188 G polyclonal mouse myeloma cell line NS0 derived recombinant protein Pro59 - Asp414 WB, FACS, ICC, IHC, IP Santa Cruz Biotechnology H-11 M monoclonal aa 41-160 WB, IP, IF, IHC, ELISA Sigma-Aldrich SAB1300310 R polyclonal N-terminal region WB, ELISA M – mouse, R – rabbit, G – goat, WB – Wester blotting, FACS – fluorescence-activated cell sorting, IHC – immunohistochemistry, IF – immunofluorescence, ICC – immunocytochemistry, ELISA – enzyme-linked immunosorbent assay, IP – immunoprecipitation, ChIP – chromatin immunoprecipitation Supplementary Table S2: Dilutions of all anti-CA IX antibodies selected on the basis of manufacturers’ recommendation and authors’ experience.

    Immunofluorescence:

    Article Title: Understanding metabolic alterations and heterogeneity in cancer progression through validated immunodetection of key molecular components: a case of carbonic anhydrase IX
    Article Snippet: Name Host Antibody type Immunogen/epitope Key applications M75 M monoclonal PG region WB, FACS, IP, IF, IHC, ELISA Abcam ab15086 R polyclonal synthetic peptide (aa 359-459) WB, FACS, IHC Cell Signaling Technology D10C10 R monoclonal synthetic peptide (around Asp361) WB, IHC Invitrogen/ThermoFisher Scientific GT12 M monoclonal linear repetitive epitope in the PG region WB, FACS, IF, ICC, IHC, IP Merck 2D3 M monoclonal purified recombinant fragment expressed in E. coli WB, FACS, IHC Novus Biologicals NB100417 R polyclonal synthetic peptide from the Cterminal region (aa 400-459) WB, FACS, IF, ICC, IHC, ELISA, ChIP, Gel Supershift, R&D Systems AF2188 G polyclonal mouse myeloma cell line NS0 derived recombinant protein Pro59 - Asp414 WB, FACS, ICC, IHC, IP Santa Cruz Biotechnology H-11 M monoclonal aa 41-160 WB, IP, IF, IHC, ELISA Sigma-Aldrich SAB1300310 R polyclonal N-terminal region WB, ELISA M – mouse, R – rabbit, G – goat, WB – Wester blotting, FACS – fluorescence-activated cell sorting, IHC – immunohistochemistry, IF – immunofluorescence, ICC – immunocytochemistry, ELISA – enzyme-linked immunosorbent assay, IP – immunoprecipitation, ChIP – chromatin immunoprecipitation Supplementary Table S2: Dilutions of all anti-CA IX antibodies selected on the basis of manufacturers’ recommendation and authors’ experience.

    Immunoprecipitation:

    Article Title: Understanding metabolic alterations and heterogeneity in cancer progression through validated immunodetection of key molecular components: a case of carbonic anhydrase IX
    Article Snippet: Name Host Antibody type Immunogen/epitope Key applications M75 M monoclonal PG region WB, FACS, IP, IF, IHC, ELISA Abcam ab15086 R polyclonal synthetic peptide (aa 359-459) WB, FACS, IHC Cell Signaling Technology D10C10 R monoclonal synthetic peptide (around Asp361) WB, IHC Invitrogen/ThermoFisher Scientific GT12 M monoclonal linear repetitive epitope in the PG region WB, FACS, IF, ICC, IHC, IP Merck 2D3 M monoclonal purified recombinant fragment expressed in E. coli WB, FACS, IHC Novus Biologicals NB100417 R polyclonal synthetic peptide from the Cterminal region (aa 400-459) WB, FACS, IF, ICC, IHC, ELISA, ChIP, Gel Supershift, R&D Systems AF2188 G polyclonal mouse myeloma cell line NS0 derived recombinant protein Pro59 - Asp414 WB, FACS, ICC, IHC, IP Santa Cruz Biotechnology H-11 M monoclonal aa 41-160 WB, IP, IF, IHC, ELISA Sigma-Aldrich SAB1300310 R polyclonal N-terminal region WB, ELISA M – mouse, R – rabbit, G – goat, WB – Wester blotting, FACS – fluorescence-activated cell sorting, IHC – immunohistochemistry, IF – immunofluorescence, ICC – immunocytochemistry, ELISA – enzyme-linked immunosorbent assay, IP – immunoprecipitation, ChIP – chromatin immunoprecipitation Supplementary Table S2: Dilutions of all anti-CA IX antibodies selected on the basis of manufacturers’ recommendation and authors’ experience.



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